GPR113 is a gene that encodes the Probable G-protein coupled receptor 113 protein.[5][6]
| ADGRF3 | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| Aliases | ADGRF3, PGR23, hGPCR37, GPR113, adhesion G protein-coupled receptor F3 | ||||||||||||||||||||||||||||||||||||||||||||||||||
| External IDs | MGI: 2685887; HomoloGene: 17826; GeneCards: ADGRF3; OMA:ADGRF3 - orthologs | ||||||||||||||||||||||||||||||||||||||||||||||||||
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Gene
editThe human GPR113 gene is located on chromosome 2 (2p23.3). This gene spans the length of a 38.65kb region from base 26531041 to 26569685 on the negative strand.[7] The GPR113 gene has two neighbors on either side on the negative strand: OTOF otoferlin preceding and HADHA hydroxyacyl-CoA following. Directly opposite the GPR113 on the positive strand is the EPT1 gene. The GPR113 gene is also known by the aliases PGR23 and HGPCR37.[8]
Evolution
editThe GPR113 has 5 human paralogs GPR110, GPR115, GPR128, GPR111, and GPR116.[8] GPR113 is well conserved in mammals from primates to semi-aquatic species, as well as some amphibians. These include the Common Chimpanzee, the African Bush Elephant, the Platypus, and the Western Clawed Frog.[9] Homologous domains that are well conserved throughout orthologs center in the 7 transmembrane receptor (Secretin family) region highlighted in purple in the figure.[10]
Protein
edit
The protein product of GPR113 gene is a G-protein coupled receptor. The protein has three transcript variants in humans.[11] Of these three, GPR113 Variant 1 has the longest amino acid sequence, and has the highest identity to orthologs. This leads to the conclusion that GPR113 Variant 1 is the homo sapiens descendant of the ancestral GPR113 gene. GPR113 Var 1 contains 1079 Amino Acids, and is integral to the plasma membrane.[12] The 7-pass receptor contains 4 domains highlighted in the figure at right: Signal Peptide (Red), Hormone Receptor Domain (Blue), Latrophilin/CL-1-like GPS domain (Orange), and the 7-transmembrane receptor (Purple). Between the Hormone Receptor Domain and the GPS is a Domain of unknown function that is not highlighted.
Function
editGPR113 is a G protein-coupled receptor that is involved in a neuropeptide signaling pathway.[12]
Clinical signficance
editGPR113 has been found to be expressed differentially under diseased conditions. Under the condition of Type 2 diabetes, the percentile rank relative to other transcripts decreases relative to normal cell function.[13] The deletion of TP63, which mediates a wide variety of important body processes, also produces decreased GPR113 expression.[14] In mice brains, the cerebellum and the olfactory bulb both show transcription of the GPR113 gene.[15] Additionally, a study from the National Institute of Deafness and Other Communication Disorders has identified GPR113 expression to be highly restricted to a subset of taste receptor cells.[16] This paper's conclusions, coupled with olfactory bulb expression levels, could provide an avenue for future research, potentially illuminating more about GPR113's function.
Interacting protein
editGPR113 has been shown to associate with the orphan G protein-coupled receptor GPR123.[17]
Transcription factors
edit| Top 10 Transcription factors of highest likelihood[18] |
|---|
| Binding site for S8 type homeodomains |
| Binding site for S8 type homeodomains |
| DLX-1, -2, and -5 binding sites |
| TCF/LEF-1 (secondary DNA binding preference) |
| Homeobox containing germ cell-specific transcription factor NOBOX |
| Spalt-like transcription factor 1 |
| Transcriptional repressor CDP |
| Alternative splicing variant of FOXP1, activated in ESCs |
| Binding site for S8 type homeodomains |
| Homeobox containing germ cell-specific transcription factor NOBOX |
Clinical significance
editThe clinical significance of this protein has not been established. However, the expression profiles provide exciting directions for future research of the GPR113 gene, especially in fields studying taste and smell.
References
edit- ^ a b c GRCh38: Ensembl release 89: ENSG00000173567 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000067642 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Fredriksson R, Lagerström MC, Höglund PJ, Schiöth HB (November 2002). "Novel human G protein-coupled receptors with long N-terminals containing GPS domains and Ser/Thr-rich regions". FEBS Letters. 531 (3): 407–414. doi:10.1016/S0014-5793(02)03574-3. PMID 12435584. S2CID 7449692.
- ^ "Entrez Gene: GPR113 G protein-coupled receptor 113".
- ^ "Homo sapiens chromosome 2, GRCh37.p13 Primary Assembly". Nucleotide. U.S. National Library of Medicine. 2013-08-13.
- ^ a b "ADGRF3 Gene - GeneCards | AGRF3 Protein | AGRF3 Antibody".
- ^ "BLAST: Basic Local Alignment Search Tool".
- ^ "SDSC Biology Workbench".
- ^ "Homo sapiens adhesion G protein-coupled receptor F3 (ADGRF3), transcript variant 2, mRNA". Nucleotide. U.S. National Library of Medicine. 2018-06-24.
- ^ a b "ADGRF3 - Adhesion G-protein coupled receptor F3 precursor - Homo sapiens (Human) - ADGRF3 gene & protein".
- ^ "ADGRF3 - Type 2 diabetes and role of hepatokines". GEO Profiles. U.S. National Center for Biotechnology Information (NCBI). 71224040.
- ^ "ADGRF3 - p63 depletion effect". GEO Profiles. U.S. National Center for Biotechnology Information (NCBI). 34655140.
- ^ "G protein-coupled receptor 113". Allen Brain Atlas: Mouse Brain.
- ^ LopezJimenez ND, Sainz E, Cavenagh MM, Cruz-Ithier MA, Blackwood CA, Battey JF, et al. (April 2005). "Two novel genes, Gpr113, which encodes a family 2 G-protein-coupled receptor, and Trcg1, are selectively expressed in taste receptor cells". Genomics. 85 (4): 472–482. doi:10.1016/j.ygeno.2004.12.005. PMID 15780750.
- ^ "STRING: Functional protein association networks".
- ^ "ElDorado Introduction". Archived from the original on 2021-05-22. Retrieved 2013-05-12.
Further reading
edit- Takeda S, Kadowaki S, Haga T, Takaesu H, Mitaku S (June 2002). "Identification of G protein-coupled receptor genes from the human genome sequence". FEBS Letters. 520 (1–3): 97–101. doi:10.1016/S0014-5793(02)02775-8. PMID 12044878. S2CID 7116392.
- Vassilatis DK, Hohmann JG, Zeng H, Li F, Ranchalis JE, Mortrud MT, et al. (April 2003). "The G protein-coupled receptor repertoires of human and mouse". Proceedings of the National Academy of Sciences of the United States of America. 100 (8): 4903–4908. Bibcode:2003PNAS..100.4903V. doi:10.1073/pnas.0230374100. PMC 153653. PMID 12679517.
- Bjarnadóttir TK, Fredriksson R, Höglund PJ, Gloriam DE, Lagerström MC, Schiöth HB (July 2004). "The human and mouse repertoire of the adhesion family of G-protein-coupled receptors". Genomics. 84 (1): 23–33. doi:10.1016/j.ygeno.2003.12.004. PMID 15203201.