Sequencing

In genetics, sequencing means to determine the nucleotides of a DNA strand. Methods to do this are :

• chromosome walking
• shotgun sequencing
• Chain termination method

In biochemistry, sequencing means more generally the determination of the primary sequence of any heterobiopolymer. This includes nucleotide sequencing as well as protein sequencing (using Edman degradation, mass spectrometry, protease digests), though it is not common to talk of sequencing a polysaccharide.