Talk:Shotgun sequencing

Deleted

The DNA is first cut into small pieces by restriction enzymes.

because I feel certain that's wrong. If the DNA is cut, it's more likely fractionated, because it's important to get random samples. The article as a whole is pretty vague and somewhat suspect, but unfortunately I don't know that much about the details either...

Zashaw 23:11, 21 Jun 2004 (UTC)

So how do you do the sequencing then?

There's something odd about this article: Sequencing lists this as a method used to sequence DNA, but this article says "...and then these clones are sequenced". How can the last step of sequencing be sequencing? Does it mean, "these clones are sequenced using sequencing method X"? Of the three methods mentioned under sequencing, only Chain termination method seems to actually be a method of sequencing, the other two just refer back to some unexplained "sequencing" process. Anyone care to clarify? - IMSoP 00:19, 22 Oct 2004 (UTC)

Fixed (I hope I got it right). - IMSoP 21:21, 14 Nov 2004 (UTC)

Whole genome shotgun sequencing

What is the difference between this and Whole genome shotgun sequencing? Jmeppley 21:26, 26 May 2005 (UTC)[reply]

The two articles should really be merged. An alternative to WGS is the BAC-based method...the difference is in how careful you are about splitting up the original DNA into pieces to be sequenced. But the basic idea of assembly is the same with either method, and the term "shotgun" is in contrast to chromosome walking. The simplified information presently in this article applies to both WGS and BAC approaches. (see for example [1]) --Mike Lin 08:54, 13 October 2005 (UTC)[reply]