Fura-2: Difference between revisions

| verifiedrevid = 400096678

| Name = '''Fura-2'''

| ImageFile = Fura-2.svg

| ImageSize = 200px

| ImageName = Fura-2

| Section1 = {{Chembox Identifiers

| CASNo = 113694-64-7

| Section2 = {{Chembox Properties

| Formula = '''[[Carbon|C]]'''₂₉'''[[Hydrogen|H]]'''₂₂'''[[Nitrogen|N]]'''₃'''[[Oxygen|O]]'''₁₄^5-

| MolarMass = 636.50 g/mol

'''Fura-2''', a [[polyamino carboxylic acid]], is a ratiometric [[fluorescent]] dye which binds to free intracellular [[calcium]].<ref>{{cite journal | author=Grynkiewicz, G., Poenie, M., and Tsien, R.Y. | title=A new generation of Ca2+ indicators with greatly improved fluorescence properties* | journal=J. Biol. Chem. | volume=260 | pages=3440–3450 | year=1985 | url=http://tsienlab.ucsd.edu/Publications/Grynkiewicz%201985%20JBC%20-%20New%20Generation%20Ca2+%20Indicators.pdf | pmid=3838314 | issue=6}}</ref> It was the first widely-used dye for [[calcium imaging]], and remains very popular. Fura-2 is excited at 340&nbsp;[[nanometer|nm]] and 380&nbsp;nm of light, and the ratio of the emissions at those wavelengths is directly correlated to the amount of intracellular calcium. Regardless of the presence of calcium, Fura-2 emits at 510&nbsp;nm of light. The use of the ratio automatically cancels out confounding variables, such as variable dye concentration and cell thickness, making Fura-2 one of the most appreciated tools to quantify calcium levels. More recently, genetically-encoded calcium indicators based on spectral variants of the [[green fluorescent protein]], such as [[Cameleon (protein)|Cameleon]]s,<ref>{{cite journal |author=Miyawaki A, Llopis J, Heim R, ''et al.'' |title=Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin |journal=Nature |volume=388 |issue=6645 |pages=882–7 |year=1997 |pmid=9278050 |doi=10.1038/42264}}</ref> have supplemented the use of Fura-2 and other small molecule dyes for calcium imaging.

* [[Fura-2AM]], a membrane-permeable derivative of Fura-2

==External links==

* [http://www.fura-2.com Fura-2 Information site]

<references/>

[[Category:Benzofurans]]

[[Category:Phenol ethers]]

[[de:Fura-2]]