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Podjedinica 6 integratorskog kompleksa je protein koji je kod ljudi kodiran genom INTS6 , koji je lociran na hromosomu 13, sekvenca 13q14.3 , u rasponu bazbih parova od 51,354.077 do 51,454.264 bp[ 5] [ 6] [ 7]
Proteini DEAD-kutija , karakterizirani konzerviranim motivom Asp-Glu-Ala-Asp (DEAD), pretpostavljene su RNK -helikaze . Protein kodiran ovim genom je DEAD boksni protein, koji je dio kompleksa koji stupa u interakciju sa C-terminalom RNK-polimeraze II i uključen je u 3' završnu preradu snRNK. Osim toga, ovaj gen je kandidat kao supresorski gen tumora i nalazi se u kritičnoj regiji gubitka heterozigotnosti (LOH). Za ovaj gen su pronađene tri varijante transkripta koje kodiraju dvije različite izoforme .[ 7]
Dužina polipeptidnog lanca je 887 aminokiselina , a molekulska težina 100.390 Da .[ 8]
10 20 30 40 50 MPILLFLIDT SASMNQRSHL GTTYLDTAKG AVETFMKLRA RDPASRGDRY MLVTFEEPPY AIKAGWKENH ATFMNELKNL QAEGLTTLGQ SLRTAFDLLN LNRLVTGIDN YGQGRNPFFL EPAIIITITD GSKLTTTSGV QDELHLPLNS PLPGSELTKE PFRWDQRLFA LVLRLPGTMS VESEQLTGVP LDDSAITPMC EVTGGRSYSV CSPRMLNQCL ESLVQKVQSG VVINFEKAGP DPSPVEDGQP DISRPFGSQP WHSCHKLIYV RPNPKTGVPI GHWPVPESFW PDQNSPTLPP RTSHPVVKFS CTDCEPMVID KLPFDKYELE PSPLTQFILE RKSPQTCWQV YVSNSAKYSE LGHPFGYLKA STALNCVNLF VMPYNYPVLL PLLDDLFKVH KAKPTLKWRQ SFESYLKTMP PYYLGPLKKA VRMMGAPNLI ADSMEYGLSY SVISYLKKLS QQAKIESDRV IGSVGKKVVQ ETGIKVRSRS HGLSMAYRKD FQQLLQGISE DVPHRLLDLN MKEYTGFQVA LLNKDLKPQT FRNAYDIPRR NLLDHLTRMR SNLLKSTRRF LKGQDEDQVH SVPIAQMGNY QEYLKQVPSP LRELDPDQPR RLHTFGNPFK LDKKGMMIDE ADEFVAGPQN KHKRPGEPNM QGIPKRRRCM SPLLRGRQQN PVVNNHIGGK GPPAPTTQAQ PDLIKPLPLH KISETTNDSI IHDVVENHVA DQLSSDITPN AMDTEFSASS PASLLERPTN HMEALGHDHL GTNDLTVGGF LENHEEPRDK EQCAEENIPA SSLNKGKKLM HCRSHEEVNT ELKAQIMKEI RKPGRKYERI FTLLKHVQGS LQTRLIFLQN VIKEASRFKK RMLIEQLENF LDEIHRRANQ INHINSN
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^ a b c GRCh38: Ensembl release 89: ENSG00000102786 - Ensembl , maj 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000035161 - Ensembl , maj 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Wieland I, Arden KC, Michels D, Klein-Hitpass L, Bohm M, Viars CS, Weidle UH (Oct 1999). "Isolation of DICE1: a gene frequently affected by LOH and downregulated in lung carcinomas" . Oncogene . 18 (32): 4530–7. doi :10.1038/sj.onc.1202806 PMID 10467397 . ^ Baillat D, Hakimi MA, Naar AM, Shilatifard A, Cooch N, Shiekhattar R (Oct 2005). "Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II" . Cell . 123 (2): 265–76. doi :10.1016/j.cell.2005.08.019 PMID 16239144 . ^ a b "Entrez Gene: INTS6 integrator complex subunit 6" .^ "UniProt, Q9UL03" . Pristupljeno 11. 9. 2017 .
Buchsbaum S, Morris C, Bochard V, Jalinot P (2007). "Human INT6 interacts with MCM7 and regulates its stability during S phase of the cell cycle" . Oncogene . 26 (35): 5132–44. doi :10.1038/sj.onc.1210314 PMID 17310990 . Hernández M, Papadopoulos N, Almeida TA (2006). "Absence of mutations in DICE1/DDX26 gene in human cancer cell lines with frequent 13q14 deletions". Cancer Genet. Cytogenet . 163 (1): 91–2. doi :10.1016/j.cancergencyto.2005.04.014 . PMID 16271964 . Röpke A, Buhtz P, Böhm M, et al. (2005). "Promoter CpG hypermethylation and downregulation of DICE1 expression in prostate cancer" . Oncogene . 24 (44): 6667–75. doi :10.1038/sj.onc.1208824 PMID 16007164 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 . Wieland I, Sell C, Weidle UH, Wieacker P (2005). "Ectopic expression of DICE1 suppresses tumor cell growth". Oncol. Rep . 12 (2): 207–11. doi :10.3892/or.12.2.207 . PMID 15254679 . Dunham A, Matthews LH, Burton J, et al. (2004). "The DNA sequence and analysis of human chromosome 13" . Nature . 428 (6982): 522–8. doi :10.1038/nature02379 . PMC 2665288 PMID 15057823 . Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs" . Nat. Genet . 36 (1): 40–5. doi :10.1038/ng1285 PMID 14702039 . Li WJ, Hu N, Su H, et al. (2003). "Allelic loss on chromosome 13q14 and mutation in deleted in cancer 1 gene in esophageal squamous cell carcinoma" . Oncogene . 22 (2): 314–8. doi :10.1038/sj.onc.1206098 PMID 12527901 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. doi :10.1073/pnas.242603899 . PMC 139241 PMID 12477932 . Wieland I, Röpke A, Stumm M, et al. (2002). "Molecular characterization of the DICE1 (DDX26) tumor suppressor gene in lung carcinoma cells". Oncol. Res . 12 (11–12): 491–500. PMID 11939413 . Camargo AA, Nunes DN, Samaia HB, et al. (2002). "Molecular characterization of DDX26, a human DEAD-box RNA helicase, located on chromosome 7p12" . Braz. J. Med. Biol. Res . 34 (10): 1237–45. doi :10.1590/s0100-879x2001001000002 PMID 11593297 . 
