METTL1
tRNK (guanin-N(7)-)-metiltransferaza je enzim koji je kod ljudi kodiran genom METTL1.[5][6]
Ovaj gen sličan je genu YDL201w Saccharomyces cerevisiae. Proizvod ovog gena sadrži konzervirani S-adenozil-metionin-vezujući motiv i inaktivira se putem fosforilacije. Alternativno prerađene varijante kodiraju različite izoforme primjenjujućih alternativnih mjesta polyA koja su opisana u literaturi.[6]
Aminokiselinska sekvenca
[uredi | uredi izvor]Dužina polipeptidnog lanca je 276 aminokiselina, a molekulska težina 3. 471 Da.[7]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MAAETRNVAG | AEAPPPQKRY | YRQRAHSNPM | ADHTLRYPVK | PEEMDWSELY | ||||
| PEFFAPLTQN | QSHDDPKDKK | EKRAQAQVEF | ADIGCGYGGL | LVELSPLFPD | ||||
| TLILGLEIRV | KVSDYVQDRI | RALRAAPAGG | FQNIACLRSN | AMKHLPNFFY | ||||
| KGQLTKMFFL | FPDPHFKRTK | HKWRIISPTL | LAEYAYVLRV | GGLVYTITDV | ||||
| LELHDWMCTH | FEEHPLFERV | PLEDLSEDPV | VGHLGTSTEE | GKKVLRNGGK | ||||
| NFPAIFRRIQ | DPVLQAVTSQ | TSLPGH |
- Simboli
C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin
Također pogledajte
[uredi | uredi izvor]Reference
[uredi | uredi izvor]- ^ a b c GRCh38: Ensembl release 89: ENSG00000037897 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000006732 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Bahr A, Hankeln T, Fiedler T, Hegemann J, Schmidt ER (Aug 1999). "Molecular analysis of METTL1, a novel human methyltransferase-like gene with a high degree of phylogenetic conservation". Genomics. 57 (3): 424–8. doi:10.1006/geno.1999.5780. PMID 10329009.
- ^ a b "Entrez Gene: METTL1 methyltransferase like 1".
- ^ "UniProt, Q9UBP6". Pristupljeno 11. 8. 2021.
Dopunska literatura
[uredi | uredi izvor]- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Alexandrov A, Martzen MR, Phizicky EM (2002). "Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA". RNA. 8 (10): 1253–66. doi:10.1017/S1355838202024019. PMC 1370335. PMID 12403464.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Izmailova E, Bertley FM, Huang Q, et al. (2003). "HIV-1 Tat reprograms immature dendritic cells to express chemoattractants for activated T cells and macrophages". Nat. Med. 9 (2): 191–7. doi:10.1038/nm822. PMID 12539042. S2CID 26145639.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Cartlidge RA, Knebel A, Peggie M, et al. (2005). "The tRNA methylase METTL1 is phosphorylated and inactivated by PKB and RSK in vitro and in cells". EMBO J. 24 (9): 1696–705. doi:10.1038/sj.emboj.7600648. PMC 1142581. PMID 15861136.
- Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.