YAF2

YAF2
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	2D9G
Identifikatori
Aliasi	YAF2
Vanjski ID-jevi	OMIM: 607534 MGI: 1914307 HomoloGene: 136401 GeneCards: YAF2
Lokacija gena (čovjek)
Hrom.	Hromosom 12 (čovjek)
Kraj	42,238,349 bp
Lokacija gena (miš)
Hrom.	Hromosom 15 (miš)
Kraj	93,234,816 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• GO:0001948, GO:0016582 vezivanje za proteine; • vezivanje iona metala; • GO:0001105 transcription coactivator activity; • GO:0001106 transcription corepressor activity;
Ćelijska komponenta	• jedro; • citosol; • nukleoplazma;
Biološki proces	• GO:0060469, GO:0009371 positive regulation of transcription, DNA-templated; • GO:0045996 negative regulation of transcription, DNA-templated; • GO:0009373 regulation of transcription, DNA-templated; • transcription, DNA-templated; • negative regulation of G0 to G1 transition;
	Izvori:Amigo / QuickGO
Ortolozi
	10138
	67057
	ENSG00000015153
	ENSMUSG00000022634
	Q8IY57
	Q99LW6
NM_001012424; NM_001190977; NM_001190979; NM_001190980; NM_005748;
	NM_001320080
	NM_024189
	NP_001177906; NP_001177908; NP_001177909; NP_001307009; NP_005739
	NP_077151
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

YY1-pridruženi faktor 2 je protein koji je kod ljudi kodiran genom YAF2.^[5]^[6] Gen YAF2 mapiran je na hromosomu 12q12, na osnovu poravnavanja sekvence YAF2 (GenBank BC037777) sa genomskom sekvencom (GRCh38).

Protein kodiran ovim genom stupa u interakciju s YY1, proteinom cinkovog prsta, koji je uključen u negativnu regulaciju gena ograničenih na mišiće. Sam ovaj genski proizvod sadrži jedan N-terminalni C2-X10-C2 cinkov prst, i za razliku od YY1, nsdreguliran je tokom miogene diferencijacije. Također olakšava proteolitsko cijepanje YY1 pomoću proteaze aktivirane kalcijem, m-kalpaina, sugerirajući mehanizam pomoću kojeg ovaj protein antagonizira negativaom učinku YY1.^[6]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 180 aminokiselina, a molekulska težina 19.901 Da.^[7]

10	20	30	40	50
MGDKKSPTRP	KRQPKPSSDE	GYWDCSVCTF	RNSAEAFKCM	MCDVRKGTST
RKPRPVSQLV	AQQVTQQFVP	PTQSKKEKKD	KVEKEKSEKE	TTSKKNSHKK
TRPRLKNVDR	SSAQHLEVTV	GDLTVIITDF	KEKTKSPPAS	SAASADQHSQ
SGSSSDNTER	GMSRSSSPRG	EASSLNGESH

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Kloniranje i ekspresija

Koristeći YY1 kao sondu u 2-hibridnom kvascu, Kalenik et al. (1997) klonirali YAF2 iz biblioteke cDNK mišića. Izvedeni protein od 180 aminokiselina ima izračunatu molekulsku masu od oko 20 kD. Sadrži N-terminalni motiv cinkovog prsta tipa C2-X10-C2, gdje je X bilo koja aminokiselina, i navodni signal jedarne lokalizacije. YAF2 dijeli homologiju ograničene sekvence s nekoliko nukleoporina i RAN-vezujućeg proteina-2 (RANBP2).^[8]

Prema Northern blot analizi, Sawa et al. (2002) otkrili su ekspresiju YAF2 u mnogim tkivima, s najvećom ekspresijom u srcu i skeletnim mišićima. Uočili su homologiju između YAF2 i RYBP, posebno unutar njihovih N-terminalnih domena.^[9]

Funkcija gena

Kalenik et al. (1997.) utvrdili su da dva prva cinkova prsta YY1 vežu YAF2. Razina YAF2 iRNK povećala se tokom in vitro diferencijacije ćelija skeleta i srčanog mišića pacova, za razliku od smanjene regulacije YY1. Prekomjerna ekspresija YAF2 u ćelijama mišjih mioblasta stimulirala je aktivnost miogenog promotora. Kotransfekcija i ekspresija YY1 u ovim ćelijama preokrenuli su stimulacijski učinak YAF2. YAF2 je također povećao proteolitsko cijepanje YY1 kalcija – aktiviranom proteazom m-kalpaina.

Kotransfekcijom i ekspresijom YAF2, GABPA i GABPB u ćelijama insekata, Sawa et al. (2002) utvrdili su da je YAF2 transkripcijski koaktivator za GABP.

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000015153 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000022634 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Kalenik JL, Chen D, Bradley ME, Chen SJ, Lee TC (Mar 1997). "Yeast two-hybrid cloning of a novel zinc finger protein that interacts with the multifunctional transcription factor YY1". Nucleic Acids Res. 25 (4): 843–9. doi:10.1093/nar/25.4.843. PMC 146511. PMID 9016636.
^ ^a ^b "Entrez Gene: YAF2 YY1 associated factor 2".
^ "UniProt, Q8IY57". Pristupljeno 8. 8. 2021.
^ Kalenik, J. L., Chen, D., Bradley, M. E., Chen, S.-J., Lee, T.-C. Yeast two-hybrid cloning of a novel zinc finger protein that interacts with the multifunctional transcription factor YY1. Nucleic Acids Res. 25: 843-849, 1997. PubMed: 9016636
^ Sawa, C., Yoshikawa, T., Matsuda-Suzuki, F., Delehouzee, S., Goto, M., Watanabe, H., Sawada, J., Kataoka, K., Handa, H. YEAF1/RYBP and YAF-2 are functionally distinct members of a cofactor family for the YY1 and E4TF1/hGABP transcription factors. J. Biol. Chem. 277: 22484-22490, 2002. PubMed: 11953439

Dopunska literatura

Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Mädge B, Geisen C, Möröy T, Schwab M (2003). "Yaf2 inhibits Myc biological function". Cancer Lett. 193 (2): 171–6. doi:10.1016/S0304-3835(02)00696-1. PMID 12706874.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Ogawa H, Ishiguro K, Gaubatz S, et al. (2002). "A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells". Science. 296 (5570): 1132–6. doi:10.1126/science.1069861. PMID 12004135. S2CID 34863978.
Sawa C, Yoshikawa T, Matsuda-Suzuki F, et al. (2002). "YEAF1/RYBP and YAF-2 are functionally distinct members of a cofactor family for the YY1 and E4TF1/hGABP transcription factors". J. Biol. Chem. 277 (25): 22484–90. doi:10.1074/jbc.M203060200. PMID 11953439.
Bannasch D, Mädge B, Schwab M (2001). "Functional interaction of Yaf2 with the central region of MycN". Oncogene. 20 (41): 5913–9. doi:10.1038/sj.onc.1204747. PMID 11593398.

Vanjski linkovi

YAF2 lokacija ljudskog genoma UCSC Genome Browser.

YAF2 human gene details in the UCSC Genome Browser.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000015153 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000022634 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9016636-5] Kalenik JL, Chen D, Bradley ME, Chen SJ, Lee TC (Mar 1997). "Yeast two-hybrid cloning of a novel zinc finger protein that interacts with the multifunctional transcription factor YY1". Nucleic Acids Res. 25 (4): 843–9. doi:10.1093/nar/25.4.843. PMC 146511. PMID 9016636.

[entrez-6] "Entrez Gene: YAF2 YY1 associated factor 2".

[UniProt-7] "UniProt, Q8IY57". Pristupljeno 8. 8. 2021.

[8] Kalenik, J. L., Chen, D., Bradley, M. E., Chen, S.-J., Lee, T.-C. Yeast two-hybrid cloning of a novel zinc finger protein that interacts with the multifunctional transcription factor YY1. Nucleic Acids Res. 25: 843-849, 1997. PubMed: 9016636

[9] Sawa, C., Yoshikawa, T., Matsuda-Suzuki, F., Delehouzee, S., Goto, M., Watanabe, H., Sawada, J., Kataoka, K., Handa, H. YEAF1/RYBP and YAF-2 are functionally distinct members of a cofactor family for the YY1 and E4TF1/hGABP transcription factors. J. Biol. Chem. 277: 22484-22490, 2002. PubMed: 11953439

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