GPBP1

GPBP1
Identifikatori
Aliasi	GPBP1
Vanjski ID-jevi	OMIM: 608412 MGI: 1920524 HomoloGene: 11292 GeneCards: GPBP1
Lokacija gena (čovjek)
Hrom.	Hromosom 5 (čovjek)
Kraj	57,264,679 bp
Lokacija gena (miš)
Hrom.	Hromosom 13 (miš)
Kraj	111,626,645 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• GO:0001131, GO:0001151, GO:0001130, GO:0001204 DNA-binding transcription factor activity; • vezivanje sa DNK; • GO:0001948, GO:0016582 vezivanje za proteine;
Ćelijska komponenta	• citoplazma; • intracellular membrane-bounded organelle; • jedro; • citosol; • ćelijska membrana;
Biološki proces	• GO:0060469, GO:0009371 positive regulation of transcription, DNA-templated; • GO:0009373 regulation of transcription, DNA-templated; • transcription, DNA-templated;
	Izvori:Amigo / QuickGO
Ortolozi
	65056
	73274
	ENSG00000062194
	ENSMUSG00000032745
	Q86WP2
	Q6NXH3
	NM_001127235; NM_001127236; NM_001203246; NM_022913; NM_001331037
NM_001122963; NM_028487; NM_001361923; NM_001361924; NM_001361925;
	NM_001361926
	NP_001120707; NP_001120708; NP_001190175; NP_001317966; NP_075064
NP_001116435; NP_082763; NP_001348852; NP_001348853; NP_001348854;
	NP_001348855
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Vaskulin jest protein koji je kod ljudi kodiran genom GPBP1 sa hromosoma 5.^[5]^[6]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 473 aminokiseline, а molekulska težina 53.339 Da.^[7]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MAQHDFAPAW	LNFPTPPSST	KSSLNFEKHS	ENFAWTENRY	DVNRRRHNSS
DGFDSAIGRP	NGGNFGRKEK	NGWRTHGRNG	TENINHRGGY	HGGSSRSRSS
IFHAGKSQGL	HENNIPDNET	GRKEDKRERK	QFEAEDFPSL	NPEYEREPNH
NKSLAAGVWE	YPPNPKSRAP	RMLVIKKGNT	KDLQLSGFPV	VGNLPSQPVK
NGTGPSVYKG	LVPKPAAPPT	KPTQWKSQTK	ENKVGTSFPH	ESTFGVGNFN
AFKSTAKNFS	PSTNSVKECN	RSNSSSPVDK	LNQQPRLTKL	TRMRTDKKSE
FLKALKRDRV	EEEHEDESRA	GSEKDDDSFN	LHNSNSTHQE	RDINRNFDEN
EIPQENGNAS	VISQQIIRSS	TFPQTDVLSS	SLEAEHRLLK	EMGWQEDSEN
DETCAPLTED	EMREFQVISE	QLQKNGLRKN	GILKNGLICD	FKFGPWKNST
FKPTTENDDT	ETSSSDTSDD	DDV

Funkcija

Nedavna analiza supresivne suptraktivne hibridizacije na ljudskoj RNK izvedenoj iz aterosklerotskih plakova otkrila je gene povećane regulacije u plakovima s trombom u odnosu na stabilne plakove. Klon SSH6, koji sadrži dio navodno otvorenog okvira čitanja nepoznatog proteina, dodatno je istražen. cDNK pune dužine, koja kodira protein od 473-aminokiselina (aa), identificirana je u biblioteci cDNK vaskularnih ćelija glatkih mišića (SMC). Bioinformatička pretraga sugerirala je prisustvo više varijanti SSH6 zbog alternativne prerade egzona 3.^[5]

Analiza više tkiva Northern blotom pokazala je različit obrazac ekspresije ovih varijanti, jer je sveprisutno izražena SSH6 iRNK kojoj nedostaje egzon 3, otkrivena i van navodnog vaskularnog SMC-specifičnog oblika koji sadrži egzon 3. Western blot analiza pokazala je sveprisutni protein od 35 kDa (SSH6-beta), pored proteina od 45 kDa (vaskulin), otkrivenog u vaskularnom zidu i plazmi. Analiza arterija koje pokazuju različite faze ateroskleroze pokazala je da se omjer vaskulin/SSH6-beta povećava tokom aterogeneze. Imunohistokemijska pretraga pokazala je citoplazmatsku ekspresiju produkata gena SSH6 u makrofagima, endotelnim ćelijama i SMC-ima. Ukratko, identificirna je novua IRNK/protein, vaskulin, u arterijskom zidu i plazmi. Regulirana ekspresija vaskulina u plakovima ukazuje na ulogu u aterogenezi. Štaviše, njegovo prisustvo u plazmi otvara perspektive za vaskulin kao marker za aterosklerozu.^[5]

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000062194 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000032745 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ ^a ^b ^c Bijnens AP, Gils A, Jutten B, Faber BC, Heeneman S, Kitslaar PJ, Tordoir JH, de Vries CJ, Kroon AA, Daemen MJ, Cleutjens KB (Oct 2003). "Vasculin, a novel vascular protein differentially expressed in human atherogenesis". Blood. 102 (8): 2803–10. doi:10.1182/blood-2003-01-0306. PMID 12842993.
^ "Entrez Gene: GPBP1 GC-rich promoter binding protein 1".
^ "UniProt, Q86WP2" (jezik: engleski). Pristupljeno 15. 10. 2021.

Dopunska literatura

Szafranski K, Schindler S, Taudien S, et al. (2008). "Violating the splicing rules: TG dinucleotides function as alternative 3' splice sites in U2-dependent introns". Genome Biol. 8 (8): R154. doi:10.1186/gb-2007-8-8-r154. PMC 2374985. PMID 17672918.
Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.
Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
Hsu LC, Liu S, Abedinpour F, et al. (2003). "The murine G+C-rich promoter binding protein mGPBP is required for promoter-specific transcription". Mol. Cell. Biol. 23 (23): 8773–85. doi:10.1128/MCB.23.23.8773-8785.2003. PMC 262660. PMID 14612417.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000062194 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000032745 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid12842993-5] Bijnens AP, Gils A, Jutten B, Faber BC, Heeneman S, Kitslaar PJ, Tordoir JH, de Vries CJ, Kroon AA, Daemen MJ, Cleutjens KB (Oct 2003). "Vasculin, a novel vascular protein differentially expressed in human atherogenesis". Blood. 102 (8): 2803–10. doi:10.1182/blood-2003-01-0306. PMID 12842993.

[entrez-6] "Entrez Gene: GPBP1 GC-rich promoter binding protein 1".

[UniProt-7] "UniProt, Q86WP2" (jezik: engleski). Pristupljeno 15. 10. 2021.

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